Comments on: Problem: sequencing a heterologous mix of 16s PCR products http://has100ideas.com/idea/sequencing-heterologous-mix-of-16s-pcr-products At least one each year Sun, 11 Jul 2010 08:21:11 +0000 hourly 1 http://wordpress.org/?v=3.0 By: Tito http://has100ideas.com/idea/sequencing-heterologous-mix-of-16s-pcr-products/comment-page-1#comment-10 Tito Fri, 29 May 2009 13:58:12 +0000 http://has100ideas.com/?p=49#comment-10 <p>Hi Mac, What do you think about taking your PCR product and then digesting it with a few enzymes? After electrophoresis take that image and compare it to a virtual electrophoresis run of 1000 bacterial species, and see which bands your sample has. This would work best with probably under a dozen species at once, beyond that I imagine there will be a ton of bands to figure out . Any obvious holes in this idea?</p> <p>Titol</p> Hi Mac, What do you think about taking your PCR product and then digesting it with a few enzymes? After electrophoresis take that image and compare it to a virtual electrophoresis run of 1000 bacterial species, and see which bands your sample has. This would work best with probably under a dozen species at once, beyond that I imagine there will be a ton of bands to figure out . Any obvious holes in this idea?

Titol

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